Chinese Journal of Natural Medicines  2019, Vol. 17Issue (2): 149-154  
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LUO Xiao-Wei, LIN Yun, LU Yong-Jun, ZHOU Xue-Feng, LIU Yong-Hong. Peptides and polyketides isolated from the marine sponge-derived fungus Aspergillus terreus SCSIO 41008[J]. Chinese Journal of Natural Medicines, 2019, 17(2): 149-154.
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Research funding

This work was supported by the National Natural Science Foundation of China (Nos. 41476135, 21772210, and 81741154) and Guangdong Special Support Projects (Leading talent for LIU Yong-Hong, and Young talent for ZHOU Xue-Feng)

Corresponding author

Tel: 86-20-89023174, E-mail: xfzhou@scsio.ac.cn (ZHOU Xue-Feng)
Tel: 86-20-89023244, E-mail: yonghongliu@scsio.ac.cn (LIU Yong-Hong)

Article history

Received on: 17-Jul-2018
Available online: 20 February, 2019
Peptides and polyketides isolated from the marine sponge-derived fungus Aspergillus terreus SCSIO 41008
LUO Xiao-Wei1,2 , LIN Yun3,4 , LU Yong-Jun3,4 , ZHOU Xue-Feng1,2 , LIU Yong-Hong1,2     
1 CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China;
2 University of Chinese Academy of Sciences, Beijing 100049, China;
3 School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China;
4 Biomedical Center of Sun Yat-Sen University, Guangzhou 510275, China
[Abstract]: Two new isomeric modified tripeptides, aspergillamides C and D (compounds 1 and 2), together with fifteen known compounds (compounds 3-17), were obtained from the marine sponge-derived fungus Aspergillus terreus SCSIO 41008. The structures of the new compounds, including absolute configurations, were determined by extensive analyses of spectroscopic data (NMR, MS, UV, and IR) and comparisons between the calculated and experimental electronic circular dichroism (ECD) spectra. Butyrolactone I (compound 11) exhibited strong inhibitory effects against Mycobacterium tuberculosis protein tyrosine phosphatase B (MptpB) with the IC50 being 5.11 ± 0.53 μmol·L-1, and acted as a noncompetitive inhibitor based on kinetic analysis.
[Key words]: Aspergillus terreus     Aspergillamides     Polyketides     MptpB inhibitor    
Introduction

Marine-derived peptides possess high potential nutraceutical and medicinal applications, due to their wide range of bioactivities, such as antimicrobial, antiviral, antitumor, and antioxidative activities [1-2]. Aspergillamides, a kind of modified tripeptides possessing rare dehydrotrytamine moieties, are mainly isolated from the marine-derived Aspergillus fungi with modest cytotoxicities, demonstrating structural diversity arising from geometric isomerization of double bonds and various categories of amino acid [3-4]. However, no more than ten natural aspergillamides have been reported. Marine sponge-associated microorganisms have been proven to be a prolific source of secondary metabolites with novel structures and interesting bioactivities [5-7]. The fungus Aspergillus terreus, a well-known producer of lovastatin [8], can produce a variety of structural classes, such as butenolides [9-11], terpenoids [12-13], peptides [14-15], and alkaloids [15-16]. Some of the above metabolites exhibit cytotoxic [10, 15, 17], NO inhibitory [9], COX-2 inhibitory [12], and antiviral activities [15].

Tuberculosis (TB) is a prevalent infectious disease caused by Mycobacterium tuberculosis (Mtb), ranking as the second deadliest communicable disease worldwide. The Mtb protein tyrosine phosphatase B (MptpB), which is secreted by Mtb into the host cell and attenuates host immune defenses by manipulating the host-signaling pathways [18], has been proven to be an important virulence factor. Recently, there has been an urgent need for finding new MptpB inhibitors with a different mode of action from currently applied drugs [19]. As our ongoing efforts for searching novel and bioactive secondary metabolites from marine microorganisms [20-22], the Aspergillus terreus SCSIO 41008 was selected for chemical investigations, owing to an interesting HPLC-UV profile for its extract cultured in a rich nutrient-culture medium by a fermenter.

Results and Discussion

Compound 1 (Fig. 1) was obtained as colorless oil. It gave a molecular formula of C28H34N4O4 as determined by the quasi-molecular ion peak at m/z 513.2482 [M + Na]+ (Calcd. 513.2478) observed from the HR-ESI-MS data. The 1H NMR spectrum (Table 1) along with HSQC experiment of 1 showed eleven aromatic or olefinic (δH 6.16‒7.63), three methinic [δH 4.85 (overlapped, H-12), 4.73 (d, J = 11.2 Hz, H-20), 2.04 (m, H-21)], two methylenic [δH 2.83 (dd, J = 9.1, 13.3 Hz, H-13a), 2.78 (dd, J = 6.3, 13.3 Hz, H-13b), 1.40 (m, H-22a), 0.98 (m, H-22b)], and four methyl [two singlets (δH 2.63 for H3-18 and δH 1.93 for H3-26), one doublet (δH 0.93, d, J = 6.3 Hz, H3-24), and one triplet (δH 0.85, t, J = 6.3 Hz, H3-23)] proton signals. Apart from the above 20 corresponding hydrogen-bearing carbons, eight carbons remained in the 13C NMR spectrum, including three carbonyls and five olefinics (one oxygenated).

Fig. 1 Structures of compounds 1‒17
Table 1 1H NMR (700 MHz) and 13C NMR (175 MHz) data of compounds 1 and 2 in CD3OD (J in Hz)

The aforementioned NMR characteristics showed great similarity to that of aspergillamide A (3), a modified tripeptide we also obtained from a marine fungus of the genus Aspergillus [3]. The major differences included the presence of hydroxy-substituted aromatic carbon at C-17 (δC 157.4) and one 1-methylpropyl group at C-20 in 1 instead of an aromatic methine (δH/C 7.13/127.9) and an isobutyl group in 3, respectively, which were also supported by the HMBC correlations (Fig. 2) from H-15/H-16 to C-17, from H3-24 to C-20/C-21/ C-22, and from H3-23 to C-21/C-22, as well as signals of a spin system of CH3-24/CH-21/CH2-22/CH3-23 and a AA′XX′ coupling system observed from the 1H-1H COSY data (Fig. 2). The Z configuration of Δ9 was assigned by the cis-coupling constant (JH-9/H-10 = 9.8 Hz) as well as the NOE correlations between H-9 and H-10. The gross structure of compound 1 was thus established and was assigned the trivial name aspergillamide C.

Fig. 2 Key HMBC, 1H-1H COSY and NOESY correlations of compounds 1 and 2

Compound 2 was also isolated as colorless oil and shared the same molecular formula as 1 according to the HR-ESI-MS data. The highly similar spectroscopic features, including NMR, UV, and IR data, illustrated that their structures were very closely related. The major difference in the 1H NMR spectrum was that the large trans-coupling constant between H-9 and H-10 was 14.0 Hz in 2, instead of 9.8 Hz in 1, implying the E configuration of the Δ9 double bond in 2. Accordingly, compound 2, the Δ9 double bond isomer of 1, was elucidated as shown in Fig. 1 and was accorded the trivial name aspergillamide D.

It proved difficult to incontrovertibly isolate the cis- and trans-isomer of the Δ9 double bond among aspergillamides, while the trans-isomer was a more stable conformation [3-4, 23]. Thus compound 1 was likely obtained as an artifact derived from 2 under the light during the isolation process. Both compounds 1 and 2 were assigned as trans-amide relationships based on the NOE correlation of N-Me (H3-18) and α-proton (H-20), which was also supported by the similar 1H NMR data of the α-proton among these trans-amide isomers (16) [3]. Moreover, the absolute configurations of compounds 1 and 2 were determined by specific rotations and ECD data in comparison with those of the co-isolated siblings (36), in combination with biogenetic point of view. These similar specific rotations among compounds 16 as well as the nearly identical experimental ECD curves of them (Fig. 3) suggested that compounds 1 and 2 shared the same configurations of 12S, 20S with 36 of the same biosynthetic origin. In addition, the experimental ECD curves of both 1 [227 nm (negative), 209 nm (positive)] and 2 [227 nm (negative), 201 nm (positive)] showed similar cotton effects to the calculated ECD curve [233 nm (negative), 206 nm (positive)] of (12S, 20S)-2 (Fig. 3), further confirming the above assignments. However, the absolute configurations at C-21 of 1 and 2 were undetermined by acid hydrolysis experiment due to the variable structures of them.

Fig. 3 The experimental ECD curves of compounds 1−6; B): Comparison between the experimental ECD curves of compounds 1−2 and the calculated ECD curve of compound 2

The structures of 15 co-isolated known compounds (317) were identified by comparison of their spectroscopic data with those reported literatures. They were elucidated as follows: aspergillamide A (3) [3], aspergillamide B (4) [3], cis-L- phenyla-laninamide (5) [24], trans-L-phenylalaninamide (6) [24], terretrione B (7) [25], terretrione C (8) [25], cyclo-(L-Pro-L-Phe) (9) [26], brevianamide F (10) [27], butyrolactone I (11) [28], 1, 8-dihy droxy-3-methoxy-6-methylanthracene-9, 10-dione (12) [29], questin (13) [30], 1-methyl emodin (14) [31], methyl 6-acetyl-4- methoxy-5, 7, 8-trihydroxynaphthalene-2-carboxylate (15) [32], methyl 6-acetyl-4-methoxy-5, 8-dihydroxynaphthalene-2-car bo xylate (16) [32], and (S)-6, 8-dimethoxy-3-methylisochro man-1-one (17) [33], among which 17 was obtained as a new natural product.

All the obtained compounds were evaluated for their MptpB inhibitory activity. Butyrolactone I (11) showed strong inhibitory activity with an IC50 of 5.11 ± 0.53 μmol·L–1, compared to the positive control (oleanolic acid, 22.1 ± 2.4 μmol·L–1). However, the remaining compounds revealed weak inhibitory effects with inhibition rates of < 60% at a concentration of 50 μmol·L–1. Besides, all these compounds (117) exhibited weak or no cytotoxic activities towards human glioma U87 cells at a concentration of 10 μmol·L–1 and also showed weak or no protective activity against glutamate-induced toxicity in HT22 cells at a concentration of 10 μmol·L–1. In order to clarify the mechanism of MptpB inhibitory type, the enzyme kinetic experiment in vitro was further carried out. The results (Fig. 4) demonstrated that 11 acted as a noncompetitive inhibitor. While the modified tripe p tides were reported with insecticidal activity against brine shrimp, Artemia salina [4], modest cytotoxicity towards the human colon carcinoma cell line HCT-116 was observed [3].

Fig. 4 Kinetic analysis of the inhibition of MptpB by compound 11

In summary, ten peptide derivatives (110), including two new tripeptides (1 and 2), along with seven known polyketides (1117), were obtained from the sponge-derived fungus Aspergillus terreus SCSIO 41008. The structures of the pair of isomeric naturally uncommon modified tripeptides, aspergillamides C (1) and D (2), were determined by extensive analyses of spectroscopic data and ECD calculation, including their absolute configurations. Our findings would contribute to enriching chemical context of the species Aspergillus terreus and expanding the chemical diversity of aspergillamides. Moreover, butyrolactone I (11) displayed notable inhibitory activity against MptpB and acted as a noncompetitive inhibitor, implying its therapeutic potential as an anti-TB drug.

Experimental General experimental procedure

Optical rotations were measured with an MCP 500 automatic polarimeter (Anton Paar, Graz, Austria). UV spectra were recorded on a UV-2600 spectrometer (Shimadzu, Kyoto, Japan). CD spectra were measured with a Chirascan circular dichroism spectrometer (Applied Photophysics, Ltd., Leather head, UK). IR spectra were measured on IR Affinity-1 spectro meter (Shimadzu, Kyoto, Japan). HR-ESI-MS and ESI-MS spectra data were recorded on a MaXis quadrupole-time- of-flight mass spectrometer and an amaZon SL ion trap mass spectrometer (Bruker, Karlsruhe, Germany), respectively. The NMR spectra including were recorded on an Avance 500 spectrometer (Bruker, Karlsruhe, Germany) or AVANCE Ⅲ HD 700 spectrometer (Bruker, Karlsruhe, Germany) using TMS as an internal standard. Thin layer chromatography (TLC) and column chromatography were performed on plates pre-coated with silica gel GF254 (10−40 μm) and over silica gel (200−300 mesh) (Qingdao Marine Chemical Factory, Qingdao, China), C18 reversed-phase silica gel (RP-18, 150 to 200 mesh, Fuji Silysia Chemical Ltd., Japan), and Sephadex LH-20 (Amersham Biosciences, Uppsala, Sweden), respectively. All solvents used were of analytical grade (Tianjin Fuyu Chemical and Industry Factory, Tianjin, China). Semi-preparative high performance liquid chromatography was performed on a Hitachi Primaide apparatus using an ODS column (YMC-pack ODS-A, Japan, 10 mm × 250 mm, 5 μm, 2 mL·min–1). The artificial sea salt (Guangzhou Haili Aquarium Technology Company, Guangzhou, China) was used for fermentation.

Fungal materials

The fungal strain, Aspergillus terreus SCSIO 41008, was isolated from the marine sponge Callyspongia sp., which was collected from the seaside in Xuwen County, Guangdong Province, China, in August 2013. It was identified by its mor phological characteristics and ITS gene sequences (GenBank accession No. MF536093), as well as the phylogenetic tree analysis. The strain was stored on MB agar (malt extract 15 g, sea salt 10 g, and agar 15 g) slants at 4 ℃ and a voucher specimen was deposited in the CAS Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Ocea nology, Chinese Academy of Sciences, Guangzhou, China.

Fermentation and extraction

The strain A. terreus SCSIO 41008 grown on MB plates was inoculated into 300 mL of MB medium (malt extract 15 g·L–1 and sea salt 20 g·L–1, PH 7.4 before sterilization) in a 1.0 L Erlenmeyer flask (14 flasks were employed). The flasks were then incubated at 28 ℃ on a rotary shaker at 200 r·min–1 for 4 d. The 4.2-L seed culture was successively transferred into a 65-L fermenter containing 40 L of S6 medium (potato 200 g·L–1, peptone 5 g·L–1, mannitol 20 g·L–1, maltose 20 g·L–1, glucose 20 g·L–1, monosodium glutamate (MSG) 5 g·L–1, yeast extract 3 g·L–1 and sea salt 20 g·L–1, PH 7.4), and then incubated at 28 ℃, 135 r·min–1, 12 L·min–1 aseptic air, and 3.0 Mpa for 7 d. During fermentation, 100 mL samples were withdrawn from the fermentor every day and subjected to HPLC analysis.

The whole culture broth (40 L) was harvested and filtered to yield the mycelium cake and liquid broth. The mycelium cake was extracted by ultrasonication using acetone for three times. The acetone solution was evaporated under reduced pressure to afford an aqueous solution. Then the aqueous solution was extracted three times with EtOAc to produce an EtOAc solution. The liquid broth was also extracted with EtOAc for three times. Both EtOAc solutions were combined and concentrated under vacuum to yield a crude gum (50 g).

Purification

The EtOAc extract was submitted to silica gel vacuum liquid chromatography (VLC) using step gradient elution with petroleum ether‒CH2Cl2 (100 : 0, 80 : 20, 50 : 50, 20 : 80, 0 : 100, V/V) to obtain ten fractions (Frs. 1‒10) based on TLC profiles. Compound 17 (5 mg, tR 20 min) was obtained from Fr.3 (1.3 g) followed by ODS chromatography eluting with MeCN‒H2O (10%‒100%) and semipreparative HPLC (27% MeCN/H2O, 2.5 mL·min–1), respectively. Fr. 4 (2.0 g) was separated into eight subfractions (Frs. 4-1‒4-8) by semipreparative HPLC (47% MeCN‒H2O, 2.5 mL·min–1), which meanwhile afforded Compound 15 (8 mg, tR 28 min) and compound 12 (7 mg, tR 42 min). Fr.4-3 was purified by semi-preparative HPLC (41% MeCN‒H2O, 0.04% TFA, 2.5 mL·min–1) to yield 13 (7 mg, tR 30 min). Fr. 4-4 was also purified by semi-preparative HPLC (57% MeCN‒H2O, 1.8 mL·min–1) to yield 6 (2 mg, tR 13 min) and 5 (2 mg, tR 19 min). Fr. 4-7 was purified by semi-preparative HPLC (53% MeCN‒ H2O, 3 mL·min–1) to yield 4 (6.7 mg, tR 16 min) and 3 (9.5 mg, tR 22 min). Compound 14 (58 mg, tR 19 min) was obtained from Fr. 5 (1.8 g), following ODS chromatography eluting with MeCN‒H2O (10%‒100%) and semi-preparative HPLC (63% MeCN‒H2O, 1.3 mL·min–1), respectively. Fr. 6 (1.2 g) was separated into eight subfractions (Frs. 6-1‒6-8) by ODS chromatography eluting with MeCN‒H2O (10%‒100%). Compound 9 (11 mg, tR 32min) was obtained from Fr. 6-1 by semi-preparative HPLC (16% MeCN‒H2O, 2.5 mL·min–1). Fr. 6-2 was further purified by semi-preparative HPLC (21% MeCN‒H2O, 2.5 mL·min–1) to yield 10 (5 mg, tR 21 min), 7 (21.5 mg, tR 23 min), and 8 (55 mg, tR 37 min). Fr. 7 (2.5 g) was separated into nine subfractions (Frs. 7-1‒7-9) by ODS chromatography eluting with MeOH‒H2O (10%‒100%). Fr. 7-5 was further purified by semi-preparative HPLC (31% MeCN-H2O, 2.5 mL·min–1) to yield 16 (3 mg, tR 31 min). Fr. 7-6 was further purified by semi-preparative HPLC (41% MeCN-H2O, 2.5 mL·min–1) to yield 2 (6 mg, tR 17 min), 1 (4.4 mg, tR 29 min), and 11 (700 mg, tR 40 min).

Aspergillamide C (1): colorless oil, [α]D25‒54 (c 0.20, MeOH); UV (MeOH) λmax (log ε) 280 (2.83), 220 (3.30), 200 (3.63) nm; CD (0.25 mg·mL–1, MeOH) λmaxε) 209 (+8.64), 227 (−4.65) nm; IR (film) νmax 3292, 2966, 2873, 1647, 1558, 1506, 1456, 1338 cm−1; HR-ESI-MS m/z 513.2482 [M + Na]+ (Calcd. for C28H34N4NaO4, 513.2478); For 1H and 13C NMR data, see Table 1.

Aspergillamide D (2): colorless oil, [α]D25‒53 (c 0.21, MeOH); UV (MeOH) λmax (log ε) 280 (2.64), 220 (3.36), 200 (3.65) nm; ECD (0.20 mg·mL–1, MeOH) λmaxε) 201 (+10.65), 227 (−6.51) nm; IR (film) νmax 3305, 2968, 2876, 1616, 1539, 1516, 1458, 1089 cm−1; HR-ESI-MS m/z 513.2488 [M + Na]+ (Calcd. for C28H34N4NaO4, 513.2478); For 1H and 13C NMR data, see Table 1.

MptpB inhibition assay

The target enzyme MptpB was prepared according to the reported protocols [19]. In brief, the inhibition assays were performed using the 96-well microplates (Invitrogen, Carlsbad, USA) by monitoring the hydrolysis of the fluorogenic phosphatase substrate p-nitrophenyl phosphate (pNPP) (Aladdin) according to the manufacturer's instruction. IC50 of compounds with more than 60% of inhibitory activity against MptpB was determined at different concentrations using two-fold serial dilution (1.5625‒100 μmol·L–1). Oleanolic acid was used as positive control (IC50 22.1 ± 2.4 μmol·L–1). To determine the type of inhibition, different inhibitor concentrations (0, 5, and 10 μmol·L–1) and different concentrations of pNPP (0.05, 0.1, 0.2, 0.4, 0.8, 1.6, and 3.2 mmol·L–1) were performed as explained above. The type of inhibition was determined by fitting data to Lineweaver-Burk plot by double reciprocal of inhibitor concentration versus velocity. All the assays were performed in triplicate in at least three independent experiments.

Cytotoxicity bioassay

The obtained compounds (117) were also evaluated for their cytotoxic activity against human glioma U87 cells and neuroprotective activity against glutamate-induced cell death in the hippocampal neuronal HT22 cells [34-35]. In brief, the U87 cells (American Type Culture Collection, ATCC; Manassas, VA, USA) and the mouse hippocampal HT-22 cells, provided by Seoul National University (Korea), were cultured in RPMI-1640 (Gibco, New York, USA) and DMEM media (Gibco-BRL, New York, USA), respectively, supplemented with 10% fetal bovine serum (FBS), 100 U·mL–1 of penicillin and 100 µg·mL–1 of streptomycin, at 37 ℃ in a humidified atmosphere containing 5% CO2. The cells were sub-cultured once every 2 day. Cell viability was determined using MTT assays according to the reported methods [34]. Briefly, cells were seeded into a 96-well plate at a density of 4 ×103 cells/well in growth medium and cultured to about 60%–70% confluence, prior to the initiation of experimental treatment. Cells were washed three times with PBS and 10 μL of MTT solution (5 mg·mL−1 stock) was added to the cells, and then incubated for 1 h at 37 ℃. The medium was removed carefully and 150 μL of DMSO was then added to resolve the blue formazan in living cells. Optical density (OD) value was measured at 570 nm by employing a microplate reader (TECAN A-5002, Austria). All the compounds were prepared at a concentration of 10 μmol·L–1 on the preliminary screening process.

Acknowledgements

We acknowledged the Analytical Facility Center (Mr. GAO Yong-Li, Ms. SUN Ai-Jun, Dr. XIAO Zhi-Hui, and Mr. LI Chuan-Rong) of the South China Sea Institute of Oceanology for assistance in large-scale fermentations and recording of NMR and MS data.

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