CN: 32-1845/R
ISSN: 2095-6975
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GENG Zi-Kai, LI Ya-Qun, CUI Qing-Hua, DU Rui-Kun, TIAN Jing-Zhen. Exploration of the mechanisms of Ge Gen Decoction against influenza A virus infection[J]. Chinese Journal of Natural Medicines, 2019, 17(9): 650-662

Exploration of the mechanisms of Ge Gen Decoction against influenza A virus infection

GENG Zi-Kai1, LI Ya-Qun1, CUI Qing-Hua1,2, DU Rui-Kun1,2, TIAN Jing-Zhen1,2
1 School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China;
2 Collaborative Innovation Center for Antiviral Traditional Chinese Medicine, Jinan 250355, China
Abstract:
Ge Gen Decoction (GGD), a Traditional Chinese Medicine prescription, is mainly used to treat infectious respiratory diseases and can relieve the symptoms of influenza A virus (IAV) infection. However, the underlying mechanism of GGD against IAV infection remains unclear. In this study, we found that GGD had moderate anti-IAV activity in vitro. GGD was more effective when given before the viral infection and targeted the viral attachment and replication stages rather than the internalization stage. In vivo, GGD treatment reduced the virus titers of lung tissue significantly and improved the survival rate, lung index, and pulmonary histo-pathological changes in H1N1-infected mice. We observed the changes in several key immuno-related indexes in GGD administrated H1N1-infected mice with anti-IAV drug oseltamivir phosphate as the control. GGD treatment decreased the expression of TNF-α and improved Th1/Th2 immune balance to reduce the excessive immune response in H1N1-infected mice. Besides, the expression of the toll-like receptor 7 signaling pathway in H1N1-infected mice decreased after GGD treatment. Our results showed that GGD has anti-IAV activity and can modulate the immune system to relieve lung inflammation.
Key words:    Ge Gen Decoction    Traditional Chinese Medicine (TCM)    H1N1    Antiviral    Immune regulation   
Received: 2019-06-10   Revised:
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Articles by GENG Zi-Kai
Articles by LI Ya-Qun
Articles by CUI Qing-Hua
Articles by DU Rui-Kun
Articles by TIAN Jing-Zhen
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