CN: 32-1845/R
ISSN: 2095-6975
Cite this paper:
LIU Miao, LI Xi-Wen, LIAO Bao-Sheng, LUO Lu, REN Yue-Ying. Species identification of poisonous medicinal plant using DNA barcoding[J]. Chinese Journal of Natural Medicines, 2019, 17(8): 585-590

Species identification of poisonous medicinal plant using DNA barcoding

LIU Miao1,2, LI Xi-Wen2, LIAO Bao-Sheng2, LUO Lu2, REN Yue-Ying1
1 College of Chinese Medicine Materials, Jilin Agriculture University, Changchun 130118, China;
2 Institute of Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing 100700, China Available online 20 July, 2019
The aim is to select a universal DNA barcode for identifying all poisonous medicinal plants in Chinese pharmacopoeia and their poisonous related species or adulterants. We chose 4 commonly used regions as candidate DNA barcodes (ITS2, psbA-trnH, matK and rbcL) and compared their identification efficiency in 106 species from 27 families and 65 genera totally. Data analysis was performed including the information of sequence alignment, inter/intra-specific genetic distance and data distribution, identification efficiency and the situation of Neighbor-Joining (NJ) phylogenetic trees. We found ITS2 sequence region had high variation, stable genetic distance and identification efficiency relatively. The topological structure of NJ phylogenetic tree showed monophyletic. Our findings show that ITS2 can be applied as a universal barcode for identifying poisonous medicinal plants in Chinese pharmacopoeia and their poisonous related species or adulterants.
Key words:    Sequence    Poisonous herbal medicine    Genetic distance    DNA barcoding    ITS2    Blast   
Received: 2019-05-14   Revised:
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Articles by LIU Miao
Articles by LI Xi-Wen
Articles by LIAO Bao-Sheng
Articles by LUO Lu
Articles by REN Yue-Ying
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