CN: 32-1845/R
ISSN: 2095-6975
Cite this paper:
0
LIU Miao, LI Xi-Wen, LIAO Bao-Sheng, LUO Lu, REN Yue-Ying. Species identification of poisonous medicinal plant using DNA barcoding[J]. Chinese Journal of Natural Medicines, 2019, 17(8): 585-590

Species identification of poisonous medicinal plant using DNA barcoding

LIU Miao1,2, LI Xi-Wen2, LIAO Bao-Sheng2, LUO Lu2, REN Yue-Ying1
1 College of Chinese Medicine Materials, Jilin Agriculture University, Changchun 130118, China;
2 Institute of Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing 100700, China Available online 20 July, 2019
Abstract:
The aim is to select a universal DNA barcode for identifying all poisonous medicinal plants in Chinese pharmacopoeia and their poisonous related species or adulterants. We chose 4 commonly used regions as candidate DNA barcodes (ITS2, psbA-trnH, matK and rbcL) and compared their identification efficiency in 106 species from 27 families and 65 genera totally. Data analysis was performed including the information of sequence alignment, inter/intra-specific genetic distance and data distribution, identification efficiency and the situation of Neighbor-Joining (NJ) phylogenetic trees. We found ITS2 sequence region had high variation, stable genetic distance and identification efficiency relatively. The topological structure of NJ phylogenetic tree showed monophyletic. Our findings show that ITS2 can be applied as a universal barcode for identifying poisonous medicinal plants in Chinese pharmacopoeia and their poisonous related species or adulterants.
Key words:    Sequence    Poisonous herbal medicine    Genetic distance    DNA barcoding    ITS2    Blast   
Received: 2019-05-14   Revised:
Tools
PDF (537 KB) Free
Print this page
Email this article to others
Authors
Articles by LIU Miao
Articles by LI Xi-Wen
Articles by LIAO Bao-Sheng
Articles by LUO Lu
Articles by REN Yue-Ying
References:
[1] Yaman S, Indrajit SK, Sudhaldev M. Review of certain herbal poisonous materials frequently used in Ayurvedic formulation[J]. World J Ayurveda Sci, 2017, 4(7):283-289.
[2] Yang G, Jiang W, Zhang MZ, et al. Anti-atherosclerosis effect and mechanism of phlegm-removing herbs of Rhizoma Pinelliae and Pseudobudobulbus Cremastrae seu Pleione[J]. Trad Chin Drug Res Clin Pharmacol, 2013, 24(3):230-233.
[3] Hamidreza A, Amir A, Majid GM. Podophyllotoxin:a novel potential natural anticancer agent[J]. Avicenna J Phytomed, 2017, 7(4):285-29.
[4] Vanherweghem JL, Tielemans C, Abramowicz D, et al. Rapidly progressive interstitial renal fibrosis in young women:association with slimming regimen including Chinese herbs[J]. Lancet, 1993, 341(8842):387-391.
[5] Han JP, Li MN, Luo K, et al. Identification of Daturae Flos and its adulterants based on DNA barcoding technique[J]. Acta Pharm Sinic, 2011, 46(11):1408-1412.
[6] Ye HZ, Cai JZ. Identification and application if Armeniacae Semen amarum and Persicae Semen[J]. Strait Pharm J, 2008, 20(11):85-86.
[7] Chen SL, Lin Y. The Latest Identification of the Traditional Chinese Medicine[M]. China Electronic Audio and Video Publishing Company, 2013:71-72.
[8] Steven GN, Meghan G, Dhivya S, et al. DNA barcoding detects contamination and substitution in North American herbal products[J]. BMC Med, 2013, 11(1):222-234.
[9] Knowlton N. Sibling species in the sea[J]. A Rev Ecol Syst, 1993, 24:189-216.
[10] Jarman SN,Elliott NG. 2000 DNA evidence for morphological and cryptic Cenozoic speciations in the Anaspididae ‘living fossils’ from the Triassic[J]. J Evol Biol, 2000, 13:624-633.
[11] Wang L. Applied research of identification of traditional Chinese medicine and Chinese patent medicine based on DNA barcoding[D]. Peking Union Medical College, 2016.
[12] Paul DNH, Alina C, Shelley L, et al. Biological identifications through DNA barcodes[J]. Proc R Soc Biol Sci Ser B, 2003, 270(2):313-321.
[13] Chen SL, Guo BL, Zhang GJ, et al. Advances of studies on new technology and method for identifying traditional Chinese medicinal materials[J]. China J Chin Mat Med, 2012, 37(8):1043-1955.
[14] Xiwen Li, Yang Yang, Robert Henry, et al. Plant DNA barcoding:from gene to genome[J]. Biol Camb Philos Soc, 2015, 90(1):157-166.
[15] CBOL Plant Working Group. CBOL plant working group, a DNA barcode for land plants[J]. Proc Natl Acad Sci USA, 2009, 106(31):12794-12797.
[16] Peter MH, Sean WG, Damon PL. Choosing and using a plant DNA barcode[J]. PLoS One, 2011, 6(5):e19254.
[17] Ka-Lok W, Paul PHB, Pang CS. Evaluation of seven DNA barcodes for differentiating closely related medicinal Gentiana species and their adulterants[J]. Chin Med, 2013, 8(1):1-12.
[18] Chen SL, Yao H, Han JP, et al. Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species[J]. PLoS One, 2010, 5(1):e8613.
[19] Yao H, Song JY, Liu C, et al. Use of ITS2 region as the universal DNA barcode for plants and animals[J]. PLoS One, 2010, 5(10):e13102.
[20] Pang XH, Liu C, Shi LC, et al. Utility of the trnH-psbA intergenic spacer region and its combinations as plant DNA barcodes:A meta-analysis[J]. PLoS One, 2012, 7(11):e48833.
[21] Jia J, Xu ZC, Xin TY, et al. Quality control of the traditional patent medicine Yimu Wan based on SMRT sequencing and DNA barcoding[J]. Front Plant Sci, 2017, 8(5):1-11.
[22] Meyer CP, Paulay G. DNA barcoding:error rates based on comprehensive sampling[J]. PLoS Biol, 2005, 3(12):e422.
[23] Ross HA, Murugan S, Li WLS. Testing the reliability of genetic methods of species identification via simulation[J]. Syst Biol, 2008, 57(2):216-230.
[24] James H, Diaz MD, MPH TM, et al. Poisoning by herbs and plants:rapid toxidromic classification and diagnosis[J]. Wilderness Environ Med, 2016, 27(1):136-152.
[25] Wallace LJ, Boilard SMAL, Eagle SHC, et al. DNA barcodes for everyday life:routine authentication of natural health products[J]. Food Res Int, 2012, 49:446-452.
[26] Selvaraj D, Sarma RK, Sathishkumar R. Phylogenetic analysis of chloroplast matK gene from Zingiberaceae for plant DNA barcoding[J]. Bioinformation, 2008, 3(1):24-27.
[27] Hollingsworth PM, Graham SW, Little DP. Choosing and using a plant DN barcode[J]. PLoS One, 2011, 6(5):e19254.
[28] Kress WJ, Erickson DL. A two-locus global DNA barcode for land plants:the coding rbcL gene complements the non-coding trnH-psbA spacer region[J]. PLoS ONE, 2007, 2(6):e508.
[29] Maslin O, Rossarin O, Panagiotis M. Species identification approach for both raw materials and end products of herbal supplements from Tinospora species[J]. BMC Complement Altern Med, 2018, 18(1):111.
[30] Rodriguez-Trelles F, Tarrio R, Ayala FJ. Evidence for a high ancestral GC content in Drosophila[J]. Mol Biol Evol, 2000, 17(11):1710-1717.
[31] Schultz J, Wolf M. ITS2 sequence-structure analysis in phylogenetics:a how-to manual for molecular systematics[J]. Mol Phylogenet Evol, 2009, 52(2):520-523.
[32] Yu N, Wei YL, Zhu Y. Integrated approach for identifying and evaluating the quality of Marsdenia tenacissima in the medicine market[J]. PLoS One, 2018, 13(4):e0195240.
[33] Takamichi O, Thierry L, Ge WZ, et al. Evolutionary history of the sequestrate genus Rossbeevera (Boletaceae) reveals a new genus Turmalinea and highlights the utility of ITS minisatellite-like insertions for molecular identification[J]. Persoonia, 2016, 37(1):173-198.
[34] Ji Y, Fritsch PW, Li H, et al. Phylogeny and classification of Paris (Melanthiaceae) inferred from DNA sequence data[J]. Ann Bot, 2006, 98(1):245-256.
[35] Zhu Ying-jie, Chen Shi-lin, Yao Hui, et al. DNA barcoding the medicinal plants of the genus Paris[J]. Acta Pharm Sin, 2010, 45(3):376-382.
[36] Xie L, Wang YW, Guan SY, et al. Prospects and problems for identification of poisonous plants in China using DNA barcodes[J]. Biomed Environ Sci, 2014, 27(10):794-806.
[37] Wang WL. Analysis of the Separation and Identification of Fungal Contamination and Their Mycotoxin-Producing Potential on the Surface of Fifteen Traditional Herbal Medicines[D]. Guangzhou University of Chinese Medicine, 2013.
[38] Chen SL, Yao H, Han JP, et al. Principles for molecular identification of traditional Chinese materia medica using DNA barcoding[J]. China J Chin Mat Med, 2013, 38(2):141-148.

Related Articles:
1. HOU Dian-Yun, SONG Jing-Yuan, YAO Hui, HAN Jian-Ping, PANG Xiao-Hui, SHI Lin-Chun, WANG Xiao-Chen, CHEN Shi-Lin.Molecular identification of Corni Fructus and its adulterants by ITS/ITS2 sequences[J]. Chinese Journal of Natural Medicines, 2013,11(2): 121-127