CN: 32-1845/R
ISSN: 2095-6975
Cite this paper:
HAN Hao, GUO Zhi-Kai, ZHANG Bo, ZHANG Mei, SHI Jing, LI Wei, JIAO Rui-Hua, TAN Ren-Xiang, GE Hui-Ming. Bioactive phenazines from an earwig-associated Streptomyces sp.[J]. Chinese Journal of Natural Medicines, 2019, 17(6): 475-480

Bioactive phenazines from an earwig-associated Streptomyces sp.

HAN Hao1, GUO Zhi-Kai2, ZHANG Bo1, ZHANG Mei1, SHI Jing1, LI Wei1, JIAO Rui-Hua1, TAN Ren-Xiang1,3, GE Hui-Ming1
1 State Key Laboratory of Pharmaceutical Biotechnology, Institute of Functional Biomolecules, School of Life Sciences, Nanjing University, Nanjing 210023, China;
2 Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-resources, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China;
3 State Key Laboratory Cultivation Base for TCM Quality and Efficacy, Nanjing University of Chinese Medicine, Nanjing 210023, China
Three new phenazine-type compounds, named phenazines SA-SC (1-3), together with four new natural products (4-7), were isolated from the fermentation broth of an earwig-associated Streptomyces sp. NA04227. The structures of these compounds were determined by extensive analyses of NMR, high resolution mass spectroscopic data, as well as single-crystal X-ray diffraction measurement. Sequencing and analysis of the genome data allowed us to identify the gene cluster (spz) and propose a biosynthetic pathway for these phenazine-type compounds. Additionally, compounds 1-5 exhibited moderate inhibitory activity against acetylcholinesterase (AChE), and compound 3 showed antimicrobial activities against Micrococcus luteus.
Key words:    Earwig-associated actinomycete    Phenazine    Biosynthetic pathway    Antimicrobial activity    Acetylcholinesterase inhibitory activity   
Received: 2019-04-27   Revised:
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