CN: 32-1845/R
ISSN: 2095-6975
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CHEN Qi, ZHANG Ke-Xin, LI Tai-Yuan, PIAO Xuan-Mei, LIAN Mei-Lan, AN Ren-Bo, JIANG Jun. Cardamine komarovii flower extract reduces lipopolysaccharide-induced acute lung injury by inhibiting MyD88/TRIF signaling pathways[J]. Chinese Journal of Natural Medicines, 2019, 17(6): 461-468

Cardamine komarovii flower extract reduces lipopolysaccharide-induced acute lung injury by inhibiting MyD88/TRIF signaling pathways

CHEN Qi1, ZHANG Ke-Xin1, LI Tai-Yuan2, PIAO Xuan-Mei3, LIAN Mei-Lan1,2, AN Ren-Bo1, JIANG Jun1,2
1 Key Laboratory of Natural Resource of Changbai Mountain and Functional Molecules(Ministry of Education), Yanbian University, Yanji 133002, China;
2 Agricultural College, Yanbian University, Yanji 133002, China;
3 Department of Urology, College of Medicine, Chungbuk National University, Cheongju 28644, Republic of Korea
Abstract:
In the present study, we investigated anti-inflammatory effect of Cardamine komarovii flower (CKF) on lipopolysaccharide (LPS)-induced acute lung injury (ALI). We determined the effect of CKF methanolic extracts on LPS-induced pro-inflammatory mediators NO and prostaglandin E2 (PGE2), production of pro-inflammatory cytokines (IL-1β, TNF-α, and IL-6), and related protein expression levels of MyD88/TRIF signaling pathways in peritoneal macrophages (PMs). Nuclear translocation of NF-κB-p65 was analyzed by immunofluorescence. For the in vivo experiments, an ALI model was established to detect the number of inflammatory cells and inflammatory factors (IL-1β, TNF-α, and IL-6) in bronchoalveolar lavage fluid (BALF) of mice. The pathological damage in lung tissues was evaluated through H&E staining. Our results showed that CKF can decrease the production of inflammatory mediators, such as NO and PGE2, by inhibiting their synthesis-related enzymes iNOS and COX-2 in LPS-induced PMs. In addition, CKF can downregulate the mRNA levels of IL-1β, TNF-α, and IL-6 to inhibit the production of inflammatory factors. Mechanism studies indicated that CKF possesses a fine anti-inflammatory effect by regulating MyD88/TRIF dependent signaling pathways. Immunocytochemistry staining showed that the CKF extract attenuates the LPS-induced translocation of NF-kB p65 subunit in the nucleus from the cytoplasm. In vivo experiments revealed that the number of inflammatory cells and IL-1β in BALF of mice decrease after CKF treatment. Histopathological observation of lung tissues showed that CKF can remarkably improve alveolar clearance and infiltration of interstitial and alveolar cells after LPS stimulation. In conclusion, our results suggest that CKF inhibits LPS-induced inflammatory response by inhibiting the MyD88/TRIF signaling pathways, thereby protecting mice from LPS-induced ALI.
Key words:    Cardamine komarovii flower    Inflammation    MyD88    TRIF    Acute lung injury   
Received: 2019-02-23   Revised:
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Articles by CHEN Qi
Articles by ZHANG Ke-Xin
Articles by LI Tai-Yuan
Articles by PIAO Xuan-Mei
Articles by LIAN Mei-Lan
Articles by AN Ren-Bo
Articles by JIANG Jun
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