CN: 32-1845/R
ISSN: 2095-6975
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LI Da, XIAO Jian-Qi, LIU Wen-Yuan, ZHANG Chao-Feng, AKIHISA Toshihiro, ABE Masahiko, MASTERS Eliot-T., ZHAI Wei-Wei, FENG Feng, ZHANG Jie. Vitellaria paradoxa nutshells from seven sub-Saharan countries as potential herbal medicines for treating diabetes based on chemical compositions, HPLC fingerprints and bioactivity evaluation[J]. Chinese Journal of Natural Medicines, 2019, 17(6): 446-460

Vitellaria paradoxa nutshells from seven sub-Saharan countries as potential herbal medicines for treating diabetes based on chemical compositions, HPLC fingerprints and bioactivity evaluation

LI Da1, XIAO Jian-Qi1, LIU Wen-Yuan2, ZHANG Chao-Feng2, AKIHISA Toshihiro3, ABE Masahiko3, MASTERS Eliot-T.4, ZHAI Wei-Wei1, FENG Feng1,5,6, ZHANG Jie5,6
1 Jiangsu Food and Pharmaceutical Science College, Huai'an 223003, China;
2 Department of Pharmaceutical Analysis, China Pharmaceutical University, Nanjing 210009, China;
3 Research Institute for Science and Technology, Tokyo University of Science, Chiba 278-8510, Japan;
4 World Agroforestry Centre(ICRAF), Nelson Marlbolough Institute of Technology(NMIT), Nelson 7010, New Zealand;
5 School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China;
6 Key Laboratory of Biomedical Functional Materials, China Pharmaceutical University, Nanjing 211198, China
Abstract:
The aim of the study was to determine the feasibility of the Vitellaria paradoxa nutshell as a new medicinal resource for treating diabetes. A total of forty-one compounds were identified by HPLC-DAD-Q-TOF-MS and phytochemical methods in V. paradoxa nutshell methanol extract. Based on HPLC fingerprints, four characteristic constituents were quantified and the origin of twenty-eight V. paradoxa nutshells from seven sub-Saharan countries was compared, which were classified into three groups with chemometric method. Twenty-eight samples contained high total phenolic content, and exhibited moderate-higher antioxidant activity and strong α-glucosidase inhibitory activity. Furthermore, all fractions and isolated compounds were evaluated for their antioxidant and α-glucosidase inhibitory activities, and α-glucosidase inhibitory action mechanism of four characteristic constituents including protocatechuic acid, 3, 5, 7-trihydroxycoumarin, (2R, 3R)-(+)-taxifolin and quercetin was investigated via molecular docking method, which were all stabilized by hydrogen bonds with α-glucosidase. The study provided an effective approach to waste utilization of V. paradoxa nutshell, which would help to resolve waste environmental pollution and provide a basis for developing potential herbal resource for treating diabetes.
Key words:    Vitellaria paradoxa    HPLC fingerprints    Antioxidant activity    α-Glucosidase inhibitory activity    Molecular docking   
Received: 2019-03-22   Revised:
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Articles by LI Da
Articles by XIAO Jian-Qi
Articles by LIU Wen-Yuan
Articles by ZHANG Chao-Feng
Articles by AKIHISA Toshihiro
Articles by ABE Masahiko
Articles by MASTERS Eliot-T.
Articles by ZHAI Wei-Wei
Articles by FENG Feng
Articles by ZHANG Jie
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