CN: 32-1845/R
ISSN: 2095-6975
Cite this paper:
ZHAO Guang, ZHUO Yu-Zhen, CUI Li-Hua, LI Cai-Xia, CHEN Sha-Yan, LI Dan, LIU Jun-Hong, LI Di-Hua, CUI Nai-Qiang, ZHANG Shu-Kun. Modified Da-chai-hu Decoction regulates the expression of occludin and NF-κB to alleviate organ injury in severe acute pancreatitis rats[J]. Chinese Journal of Natural Medicines, 2019, 17(5): 355-362

Modified Da-chai-hu Decoction regulates the expression of occludin and NF-κB to alleviate organ injury in severe acute pancreatitis rats

ZHAO Guang1,2, ZHUO Yu-Zhen2,3, CUI Li-Hua2,3, LI Cai-Xia2,3, CHEN Sha-Yan2,4, LI Dan1,2, LIU Jun-Hong3, LI Di-Hua3, CUI Nai-Qiang1,2, ZHANG Shu-Kun2,3
1 Hepatobiliary and Pancreatic Surgery, Tianjin Nankai Hospital, Tianjin Hospital of Integrated Traditional Chinese and Western medicine, Tianjin 300100, China;
2 Nankai Clinical College, Tianjin Medical University, Tianjin 300107, China;
3 Institute of Integrated Traditional Chinese and Western medicine, Tianjin Nankai Hospital, Tianjin Hospital of Integrated Tradi-tional Chinese and Western medicine, Tianjin 300100, China;
4 Department of Clinical Laboratory, Tianjin Nankai Hospital, Tianjin Hospital of Integrated Traditional Chinese and Western medicine, Tianjin 300100, China
Modified Da-chai-hu Decoction (MDD), a traditional Chinese medicinal formulation, which was empirically generated from Da-chai-hu decoction, has been utilized to treat severe acute pancreatitis (SAP) for decades. The aim of the present study was to explore its potential organprotective mechanism in SAP. In the present study, rat SAP model was induced by retrograde injection of 3.5% sodium taurocholate into the biliopancreatic duct, MDD (23.35 g/kg body weight, twelve times the clinical dose) were orally given at 2 h before and 10 h after injection. At 12 h after model induction, blood was taken from vena cava for analysis of amylase, diamine oxidase (DAO), pulmonary surfactant protein-A (SP-A), and C-reactive protein (CRP). Histopathological change of pancre-as, ileum and lung was assayed by H&E staining, myeloperoxidase (MPO) activity were determinated using colorimetric assay, and the expressions of occludin and nuclear factor-κB (NF-κB) were detected by real-time RT-PCR and western blot, respectively. In addition, the tissue concentrations of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and monocyte chemoattractant protein-1 (MCP-1) were measured by enzyme-linked immunosorbent assay (ELISA). The results showed that in SAP rats, MDD significantly alleviated histopathological damage, depressed the MPO activity and the concentrations of TNF-α, IL-1β, and MCP-1 of pancreas, ileum and lung, and reduced the serum levels of amylase[(3283.4 ±585.5) U·L-1 vs (5626.4 ±795.1) U·L-1], DAO[(1100.1 ±334.3) U·L-1 vs (1666.4 ±525.3) U·L-1] and CRP[(7.6 ±1.2) μg·mL-1 vs (17.8 ±3.8) μg·mL-1]. However, the serum SP-A concentration[(106.1 ±16.6) pg·mL-1 vs (90.1 ±14.9) pg·mL-1] was elevated when treated SAP rats with MDD. Furthermore, MDD increased the occludin expression and reduced the NF-κB expression in pancreas, ileum and lung of SAP rats. Our findings suggested that MDD administration was an effective therapeutic approach for SAP treatment. It could up-regulate occludin expression to protect intercellular tight junction and down-regulate NF-κB expression to inhibit inflammatory reaction of pancreas, ileum and lung.
Key words:    Severe acute pancreatitis    Modified Da-chai-hu Decoction    Occludin    Nuclear factor-κB    Ileum    Lung   
Received: 2019-03-11   Revised:
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