CN: 32-1845/R
ISSN: 2095-6975
Cite this paper:
0
QIAN Hao, YU Fang-Jun, LU Dan-Yi, WU Bao-Jian, ZHANG Xing-Wang, WANG Huan, MA Zhi-Guo. Identification of poliumoside metabolites in rat plasma, urine, bile, and intestinal bacteria with UPLC/Q-TOF-MS[J]. Chinese Journal of Natural Medicines, 2018, 16(11): 871-880

Identification of poliumoside metabolites in rat plasma, urine, bile, and intestinal bacteria with UPLC/Q-TOF-MS

QIAN Hao, YU Fang-Jun, LU Dan-Yi, WU Bao-Jian, ZHANG Xing-Wang, WANG Huan, MA Zhi-Guo
College of Pharmacy, Jinan University, Guangzhou 510632, China
Abstract:
Poliumoside is representative of phenylethanoid glycosides, which are widely found in many plants. Poliumoside is also regarded as the main active component of Callicarpa kwangtungensis Chun (CK), though its oral bioavailability in rat is extremely low (0.69%) and its in vivo and in vitro metabolism has not yet been systematically investigated. In the present study, an ultra performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS) method was employed to identify the metabolites and investigate the metabolic pathways of poliumoside in rat after oral administration 1.5 g·kg-1 of poliumoside. As a result, a total of 34 metabolites (30 from urine, 17 from plasma, and 4 from bile) and 9 possible metabolic pathways (rearrangment, reduction, hydration, hydrolyzation, dehydration, methylation, hydroxylation, acetylation, and sulfation) were proposed in vivo. The main metabolite, acteoside, was quantified after incubated with rat intestinal bacteria in vitro. In conclusion, the present study systematically explored the metabolites of poliumoside in vivo and in vitro, proposing metabolic pathways that may be significant for further metabolic studies of poliumoside.
Key words:    Poliumoside    Rat    Metabolites    Intestinal bacteria    UPLC/Q-TOF-MS   
Received: 2018-01-10   Revised:
Tools
PDF (1838 KB) Free
Print this page
Email this article to others
Authors
Articles by QIAN Hao
Articles by YU Fang-Jun
Articles by LU Dan-Yi
Articles by WU Bao-Jian
Articles by ZHANG Xing-Wang
Articles by WANG Huan
Articles by MA Zhi-Guo
References: