CN: 32-1845/R
ISSN: 2095-6975
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CHENG Qi-Qing, CHENG Chun-Song, OUYANG Yue, LAO Chi-Chou, CUI Hao, XIAN Yu, JIANG Zhi-Hong, LI Wen-Jia, ZHOU Hua. Discovery of differential sequences for improving breeding and yield of cultivated Ophiocordyceps sinensis through ITS sequencing and phylogenetic analysis[J]. Chinese Journal of Natural Medicines, 2018, 16(10): 749-755

Discovery of differential sequences for improving breeding and yield of cultivated Ophiocordyceps sinensis through ITS sequencing and phylogenetic analysis

CHENG Qi-Qing1,2, CHENG Chun-Song1,3, OUYANG Yue3, LAO Chi-Chou1,3, CUI Hao3, XIAN Yu3, JIANG Zhi-Hong1,2,3, LI Wen-Jia4, ZHOU Hua1,2,3
1 State Key Laboratory of Quality Research in Chinese Medicine(Macau University of Science and Technology), Avenida Wailong, Taipa, Macau Special Administrative Region, China;
2 Macau Institute for Applied Research in Medicine and Health, Avenida Wailong, Taipa, Macau Special Administrative Region, China;
3 Faculty of Chinese Medicine, Macau University of Science and Technology, Avenida Wailong, Taipa, Macau Special Administrative Region, China;
4 Sunshine Lake Pharma Co., Ltd., Dongguan 523808, China
Abstract:
To accelerate the breeding process of cultivated Ophiocordyceps sinensis and increase its yield, it is important to identify molecular fingerprint of dominant O. sinensis. In the present study, we collected 3 batches of industrially cultivated O. sinensis product with higher yield than the others and compared their internal transcribed spacer (ITS) sequences with the wild and the reported. The ITS sequence was obtained by bidirectional sequencing and analyzed with molecular systematics as a DNA barcode for rapid and accurate identification of wild and cultivated O. sinensis collected. The ITS sequences of O. sinensis with detailed collection loci on NCBI were downloaded to construct a phylogenetic tree together with the sequences obtained from the present study by using neighbor-joining method based on their evolution relationship. The information on collection loci was analyzed with ArcGIS 10.2 to demonstrate the geographic distribution of these samples and thus to determine the origin of the dominant samples. The results showed that all wild and cultivated samples were identified as O. sinensis and all sequences were divided into seven phylogenetic groups in the tree. Those groups were precisely distributed on the map and the process of their system evolution was clearly presented. The three cultivated samples were clustered into two dominant groups, showing the correlation between the industrially cultivated samples and the dominant wild samples, which can provide references for its optimized breeding in the future.
Key words:    Ophiocordyceps sinensis    ITS    Phylogenetic relationship    Artificial cultivation    Evolution process   
Received: 2018-04-27   Revised:
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