CN: 32-1845/R
ISSN: 2095-6975
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ZHAO Yu-Nan, SHAO Xiao, OUYANG Liu-Feng, CHEN Lin, GU Ling. Qualitative detection of ginsenosides in brain tissues after oral administration of high-purity ginseng total saponins by using polyclonal antibody against ginsenosides[J]. Chinese Journal of Natural Medicines, 2018, 16(3): 175-183

Qualitative detection of ginsenosides in brain tissues after oral administration of high-purity ginseng total saponins by using polyclonal antibody against ginsenosides

ZHAO Yu-Nan1, SHAO Xiao1, OUYANG Liu-Feng2, CHEN Lin1, GU Ling3
1 Laboratory of Pathological Sciences, College of Medicine and Life, Nanjing University of Chinese Medicine, Nanjing 210046, China;
2 Laboratory of Pathological Sciences, College of Medicine, Yan'an University, Yan'an 716000, China;
3 Research Center, Basic Medical College, Nanjing University of Chinese Medicine, Nanjing 210046, China
Abstract:
Given the limited studies and conflicting findings, the transport character of ginsenosides crossing the blood–brain barrier (BBB) remains unclear. The present study was designed to qualitatively determine the distribution of ginsenosides in brain tissues after oral administration of ginseng total saponins, using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) combined with immunohistochemistry. In brain tissue homogenates, ginsenoside Rg1 was detectable and no other ginsenosides or their metabolites were found. No ginsenosides were detected in cerebrospinal fluid. Immunohistochemistry staining of brain tissue sections by using anti-ginsenoside polyclonal antibodies revealed the localization of ginsenosides in brain tissues. Furthermore, immunofluorescence double staining revealed that ginsenosides widely existed in vascular endotheliocytes and astrocytes, and in few neurons. These results indicated that Rg1 was the main component that entered the brain after oral administration of ginseng total saponins and that ginsenosides could cross the BBB, although the transport capability of ginsenosides through the BBB may be poor.
Key words:    Ginseng    Ginsenoside    Immunostaining    Mass spectrometry    Blood-brain barrier   
Received: 2017-09-29   Revised:
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Articles by ZHAO Yu-Nan
Articles by SHAO Xiao
Articles by OUYANG Liu-Feng
Articles by CHEN Lin
Articles by GU Ling
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