CN: 32-1845/R
ISSN: 2095-6975
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Ju-Suk Nam, Supriya Jagga, Ashish Ranjan Sharma, Joon-Hee Lee, Jong Bong Park, Jun-Sub Jung, Sang-Soo Lee. Anti-inflammatory effects of traditional mixed extract of medicinal herbs (MEMH) on monosodium urate crystal-induced gouty arthritis[J]. Chinese Journal of Natural Medicines, 2017, 15(8): 561-575

Anti-inflammatory effects of traditional mixed extract of medicinal herbs (MEMH) on monosodium urate crystal-induced gouty arthritis

Ju-Suk Nam1, Supriya Jagga1, Ashish Ranjan Sharma1, Joon-Hee Lee3, Jong Bong Park1, Jun-Sub Jung2, Sang-Soo Lee1
1. Institute For Skeletal Aging & Orthopedic Surgery, Hallym University-Chuncheon Sacred Heart Hospital, Chuncheon, Gangwon-do 24252, Republic of Korea;
2. Institute of Natural Medicine, College of Medicine, Hallym University, Chucheon, Gangwon-do 24252, Republic of Korea;
3. Hana Oriental Clinic, Chucnheon, Gangwon-do 24433, Republic of Korea
Abstract:
Korean oriental medicine prescription is widely used for the treatment of gouty diseases. In the present study, we investigated anti-inflammatory effects of modified Korean herbal formulation, mixed extract of medicinal herbs (MEMH), and its modulatory effects on inflammatory mediators associated with gouty arthritis. Both in vitro and in vivo studies were carried out to assess the anti-inflammatory efficacy of MEMH on monosodium urate (MSU) crystals-induced gouty inflammation. MSU crystals stimulated human chondrosarcoma cell line, SW1353, and human primary chondrocytes were treated with MEMH in vitro. The expression levels of pro-inflammatory mediators and metalloproteases were analyzed. The effect of MEMH on NFκB signaling pathway in SW1353 cells was examined. Effect of MEMH on the mRNA expression level of pro-inflammatory mediators and chemotactic factor from human monocytic cell line, THP-1, was also analyzed. The probable role of MEMH in the differentiation process of osteoblast like cells, SaOS-2, after MSU treatment was also observed. To investigate the effects of MEMH in vivo, MSU crystals-induced ankle arthritic model was established. Histopathological changes in affected joints and plasma levels of pro-inflammatory mediators (IL-1β and TNFα) were recorded. MEMH inhibited NFκB signaling pathway and COX-2 protein expression in chondrocytes. MSU-induced mRNA expressions of pro-inflammatory mediators and chemotactic cytokines were suppressed by MEMH. In MSU crystals-induced ankle arthritic mouse model, administration of MEMH relieved inflammatory symptoms and decreased the plasma levels of IL-1β and TNFα. The results indicated that MEMH can effectively inhibit the expression of inflammatory mediators in gouty arthritis, demonstrating its potential for treating gouty arthritis.
Key words:    Gout    Monosodium urate crystals    Inflammation    Chondrocyte    Oriental medicine   
Received: 2016-11-23   Revised:
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Articles by Ju-Suk Nam
Articles by Supriya Jagga
Articles by Ashish Ranjan Sharma
Articles by Joon-Hee Lee
Articles by Jong Bong Park
Articles by Jun-Sub Jung
Articles by Sang-Soo Lee
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