CN: 32-1845/R
ISSN: 2095-6975
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Abdelsamed I. Elshamy, Walaa A. El-Kashak, Heba M. I. Abdallah, Abdelrazik H. Farrag, Mahmoud I. Nassar. Soft coral Cespitularia stolonifera: New cytotoxic ceramides and gastroprotective activity[J]. Chinese Journal of Natural Medicines, 2017, 15(2): 105-114

Soft coral Cespitularia stolonifera: New cytotoxic ceramides and gastroprotective activity

Abdelsamed I. Elshamy1, Walaa A. El-Kashak1, Heba M. I. Abdallah2, Abdelrazik H. Farrag3, Mahmoud I. Nassar1
1 Natural Compounds Chemistry Department, National Research Centre, Giza 12622, Egypt;
2 Pharmacology Department, National Research Centre, Giza 12622, Egypt;
3 Pathology Department. National Research Centre, Giza 12622, Egypt
Abstract:
In the present study, a new ceramide, namely 2S, 3R-4E, 8E-2-(heptadecanoylamino)-heptadeca-4, 8-diene-1, 3-diol (1), along with four known steroids, including 24-methylcholesta-5, 24(28)-diene-3β-ol (2), 24-methylcholesta-5, 24(28)-diene-3β-acetate (3), 4-methyl-24-methylcholesta-22-ene-3-ol (4), and cholesterol, was isolated and characterized from CH2Cl2/MeOH extract of Cespitularia stolonifera. A new acetate derivative of compound 1, termed 2S, 3R-4E, 8E-2-(heptadecanoylamino)-heptadeca-4, 8-diene-1, 3-diacetate (1a), was also prepared in the present study. All the structures were established on the basis of modern spectroscopic techniques, including FT-IR, 1D, 2D-NMR, HRESI-MS, and GC-MS, in addition of chemical methods. (-)-Alloaro-madendren, ledane, (1)-alloaromadendren oxide, isoaromadendrene epoxide and (-)-caryophellen oxide were identified from the n-hexane fraction using GC-MS. The extract and the two ceramides (1) and (1a) exhibited significant cytotoxic activity against lung cancer A549 cells, while the extract and the two steroids (2) and (3) exhibited significant cytotoxic activity against breast cancer MCF-7 cells. The CH2Cl2/MeOH extract exhibited significant antiulcer activity in both ethanol and acetic acid induced ulcer models in rats, as evidenced by histopathological, histochemical, and biochemical examinations.
Key words:    Cespitularia stolonifera    Ceramide    Steroids    Anticancer leading constituents    Antiulcer activity   
Received: 2015-04-17   Revised:
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Articles by Abdelsamed I. Elshamy
Articles by Walaa A. El-Kashak
Articles by Heba M. I. Abdallah
Articles by Abdelrazik H. Farrag
Articles by Mahmoud I. Nassar
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