CN: 32-1845/R
ISSN: 2095-6975
Cite this paper:
0
YUAN Yan, PAN Shu, YANG Shi-Lin, LIU Yan-Li, XU Qiong-Ming. Antioxidant and cardioprotective effects of Ilex cornuta on myocardial ischemia injury[J]. Chinese Journal of Natural Medicines, 2017, 15(2): 94-104

Antioxidant and cardioprotective effects of Ilex cornuta on myocardial ischemia injury

YUAN Yan1, PAN Shu2, YANG Shi-Lin3, LIU Yan-Li1, XU Qiong-Ming3
1 Department of Pharmacology, College of Pharmaceutical Science, Soochow University, Suzhou 215123, China;
2 Department of Pharmaceutical Chemistry, Guizhou University, Guiyang 550025, China;
3 Department of Pharmacognosy, College of Pharmaceutical Science, Soochow University, Suzhou 215123, China
Abstract:
Previous studies have indicated that the Ilex genus exhibits antioxidant, neuroprotective, hepatoprotective, and anti-inflammatory activities. However, the pharmacologic action and mechanisms of Ilex cornuta against cardiac diseases have not yet been explored. The present study was designed to investigate the antioxidant and cardioprotective effects of Ilex cornuta root with in vitro and in vivo models. The anti-oxidative effects of the extract of Ilex cornuta root (ICR) were measured by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging and MTT assays as well as immunoassay. Furthermore, a rat model of myocardial ischemia was established to investigate the cardioprotective effect of ICR in vivo. Eight compounds were isolated and identified from ICR and exhibited DPPH free-radical scavenging activities. They also could increase cell viability and inhibit morphological changes induced by H2O2 or Na2S2O4 in H9c2 cardiomyocytes, followed by increasing the SOD activities and decreasing the MDA and ROS levels. In addition, it could suppress the apoptosis of cardiomyocytes. In the rat model of myocardial ischemia, ICR decreased myocardial infarct size and suppressed the activities of LDH and CK. Furthermore, ICR attenuated histopathological alterations of heart tissues and the MDA levels, while increasing SOD activities in serum. In conclusion, these results suggest that ICR has cardioprotective activity and could be developed as a new food supplement for the prevention of ischemic heart disease.
Key words:    Ilex cornuta    Myocardial ischemia    Antioxidant    Apoptosis   
Received: 2016-04-17   Revised:
Tools
PDF (4614 KB) Free
Print this page
Email this article to others
Authors
Articles by YUAN Yan
Articles by PAN Shu
Articles by YANG Shi-Lin
Articles by LIU Yan-Li
Articles by XU Qiong-Ming
References:

Related Articles:
1.ZHAO Yu-Rong, QU Wei, LIU Wen-Yuan, HONG Hao, FENG Feng, CHEN Han, XIE Ning.YGS40, an active fraction of Yi-Gan San, reduces hydrogen peroxide-induced apoptosis in PC12 cells[J]. Chinese Journal of Natural Medicines, 2015,13(6): 438-444