CN: 32-1845/R
ISSN: 2095-6975
Cite this paper:
WANG Lin-Yan, TANG Yu-Ping, LIU Xin, ZHU Min, TAO Wei-Wei, LI Wei-Xia, DUAN Jin-Ao. Effects of ferulic acid on antioxidant activity in Angelicae Sinensis Radix, Chuanxiong Rhizoma, and their combination[J]. Chinese Journal of Natural Medicines, 2015, 13(6): 401-408

Effects of ferulic acid on antioxidant activity in Angelicae Sinensis Radix, Chuanxiong Rhizoma, and their combination

WANG Lin-Yan1,2, TANG Yu-Ping1, LIU Xin1, ZHU Min1, TAO Wei-Wei1, LI Wei-Xia1, DUAN Jin-Ao1
1 Jingjiang Hospital of Traditional Chinese Medicine, Jingjiang 214500, China;
2 Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China
The present study aimed at exploring different roles of the same compound in different environment, using preparative HPLC, and the significance to investigating bio-active constituents in traditional Chinese medicine (TCM) on the basis of holism. In this study, the depletion of target component ferulic acid (FA) by using preparative HPLC followed by antioxidant activity testing was applied to investigate the roles of FA in Angelicae Sinensis Radix (DG), Chuanxiong Rhizoma (CX) and their combination (GX). The antioxidant activity was performed by 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity testing. FA was successfully and exclusively depleted from DG, CX, and GX, respectively. By comparing the effects of the samples, it was found that FA was one of the main antioxidant constituents in DG, CX and GX, and the roles of FA were DG> CX> GX. Furthermore, the effects of FA varied at different doses in these herbs. This study provided a reliable and effective approach to clarifying the contribution of same compound in different TCMs to their bio-activities. The role of a constituent in different TCMs might be different, and a component with the same content might have different effects in different chemical environments. Furthermore, this study also suggested the potential utilization of preparative HPLC in the characterization of the roles of multi-ingredients in TCM.
Key words:    HPLC    Ferulic acid    Angelica sinensis    Ligusticum chuanxiong    Herb pair    Antioxidant activity   
Received: 2014-06-16   Revised:
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