CN: 32-1845/R
ISSN: 2095-6975
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Rakotomalala Manda Heriniaina, DONG Jing, Praveen Kumar Kalavagunta, WU Hua-Li, YAN Dong-Sheng, SHANG Jing. Effects of six compounds with different chemical structures on melanogenesis[J]. 中国天然药物英文, 2018, 16(10): 766-773

Effects of six compounds with different chemical structures on melanogenesis

Rakotomalala Manda Heriniaina1,2, DONG Jing3,4, Praveen Kumar Kalavagunta1,2, WU Hua-Li1,2, YAN Dong-Sheng3,4, SHANG Jing1,2
1 State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China;
2 Jiangsu Key Laboratory of TCM Evaluation and Translational Research, China Pharmaceutical University, Nanjing 211198, China;
3 School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou 325035, China;
4 State Key Laboratory of Ophthalmology, Optometry and Visual Science, Wenzhou 325035, China
摘要:
Several chemical compounds can restore pigmentation in vitiligo through mechanisms that vary according to disease etiology. In the present study, we investigated the melanogenic activity of six structurally distinct compounds, namely, scopoletin, kaempferol, chrysin, vitamin D3, piperine, and 6-benzylaminopurine. We determined their effectiveness, toxicity, and mechanism of action for stimulating pigmentation in B16F10 melanoma cells and in a zebrafish model. The melanogenic activity of 6-benzy-laminopurine, the compound identified as the most potent, was further verified by measuring green fluorescent protein concentration in tyrp1 a:eGFP (tyrosinase-related protein 1) zebrafish and mitfa:eGFP (microphthalmia associated transcription factor) zebrafish and antioxidative activity. All the tested compounds were found to enhance melanogenesis responses both in vivo and in vitro at their respective optimal concentration by increasing melanin content and expression of TYR and MITF. 6-Benzyamino-purine showed the strongest re-pigmentation action at a concentration of 20 μmol·L-1 in vivo and 100 μmol·L-1 in vitro, and up-regulated the strong fluorescence expression of green fluorescent protein in tyrp1a:eGFP and mitfa:eGFP zebrafish in vitro. However, its relative anti-oxidative activity was found to be very low. Overall, our results indicated that 6-benzylaminopurine stimulated pigmentation through a direct mechanism, by increasing melanin content via positive regulation of tyrosinase activity in vitro, as well as up-regulating the expression of the green fluorescent protein in transgenic zebrafish in vivo.
关键词:    Vitiligo    Melanogenesis    Tyrosinase    MITF    Zebrafish   
收稿日期: 2017-10-28
SHANG Jing,E-mail:shangjing21cn@163.com
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