CN: 32-1845/R
ISSN: 2095-6975
引用本文:
0
LV Qi, WANG Kai, QIAO Si-Miao, DAI Yue, WEI Zhi-Feng. Norisoboldine, a natural aryl hydrocarbon receptor agonist, alleviates TNBS-induced colitis in mice, by inhibiting the activation of NLRP3 inflammasome[J]. 中国天然药物英文, 2018, 16(3): 161-174

Norisoboldine, a natural aryl hydrocarbon receptor agonist, alleviates TNBS-induced colitis in mice, by inhibiting the activation of NLRP3 inflammasome

LV Qi, WANG Kai, QIAO Si-Miao, DAI Yue, WEI Zhi-Feng
Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Nanjing 210009, China
摘要:
Although the etiology of inflammatory bowel disease is still uncertain, increasing evidence indicates that the excessive activation of NLRP3 inflammasome plays a major role. Norisoboldine (NOR), an alkaloid isolated from Radix Linderae, has previously been demonstrated to inhibit inflammation and IL-1β production. The present study was to examine the effect of NOR on colitis and the underlying mechanism related to NLRP3 inflammasome activation. Our results showed that NOR alleviated colitis symptom in mice induced by 2, 4, 6-trinitrobenzene sulfonic acid (TNBS). Moreover, it significantly reduced expressions of cleaved IL-1β, NLRP3 and cleaved Caspase-1 but not ASC in colons of mice. In THP-1 cells, NOR suppressed the expressions of NLRP3, cleaved Caspase-1 and cleaved IL-1β but not ASC induced by lipopolysaccharide (LPS) and adenosine triphosphate (ATP). Furthermore, NOR could activate aryl hydrocarbon receptor (AhR) in THP-1 cells, inducing CYP1A1 mRNA expression, and promoting dissociation of AhR/HSP90 complexes, association of AhR and ARNT, AhR nuclear translocation, XRE reporter activity and binding activity of AhR/ARNT/XRE. Both siAhR and α-naphthoflavone (α-NF) markedly diminished the inhibition of NOR on NLRP3 inflammasome activation. In addition, NOR elevated Nrf2 level and reduced ROS level in LPS-and ATP-stimulated THP-1 cells, which was reversed by either siAhR or α-NF treatment. Finally, correlations between activation of AhR and attenuation of colitis, inhibition of NLRP3 inflammasome activation and up-regulation of Nrf2 level in colons were validated in mice with TNBS-induced colitis. Taken together, NOR ameliorated TNBS-induced colitis in mice through inhibiting NLRP3 inflammasome activation via regulating AhR/Nrf2/ROS signaling pathway.
关键词:    Norisoboldine    Inflammatory bowel disease    NLRP3 inflammasome    Aryl hydrocarbon receptor   
收稿日期: 2017-12-18
WEI Zhi-Feng, DAI Yue
相关功能
PDF(9328 KB) Free
打印本文
把本文推荐给朋友
作者相关文章
LV Qi 在本刊中的所有文章
WANG Kai 在本刊中的所有文章
QIAO Si-Miao 在本刊中的所有文章
DAI Yue 在本刊中的所有文章
WEI Zhi-Feng 在本刊中的所有文章
参考文献:
[1] Gao Z, Yu C, Liang H, et al. Andrographolide derivative CX-10 ameliorates dextran sulphate sodium-induced ulcerative colitis in mice:Involvement of NF-κB and MAPK signalling pathways[J]. Int Immunopharmacol, 2018, 57:82-90.
[2] Guo W, Sun Y, Liu W, et al. Small molecule-driven mitophagy-mediated NLRP3 inflammasome inhibition is responsible for the prevention of colitis-associated cancer[J]. Autophagy, 2014, 10(6):972-985.
[3] Zhang M, Sun K, Wu Y, et al. Interactions between intestinal microbiota and host immune response in inflammatory bowel disease[J]. Front Immunol, 2017, 8:942.
[4] Naganuma M, Mizuno S, Nanki K, et al. Recent trends and future directions for the medical treatment of ulcerative colitis[J]. Clin J Gastroenterol, 2016, 9(6):329-336.
[5] Spalinger MR, Manzini R, Hering L, et al. PTPN2 regulates inflammasome activation and controls onset of intestinal inflammation and colon cancer[J]. Cell Rep, 2018, 22(7):1835-1848.
[6] Lazaridis LD, Pistiki A, Giamarellos-Bourboulis EJ, et al. Activation of NLRP3 inflammasome in inflammatory bowel disease:Differences between crohn's disease and ulcerative colitis[J]. Dig Dis Sci, 2017, 62(9):2348-2356.
[7] Lin LR, Xiao Y, Liu W, et al. Development of tissue inflammation accompanied by NLRP3 inflammasome activation in rabbits infected with Treponema pallidum strain Nichols[J]. BMC Infect Dis, 2018, 18(1):101.
[8] Dubey S, Yoon H1, Cohen MS, et al. Withaferin A associated differential regulation of inflammatory cytokines[J]. Front Immunol, 2018, 9:195.
[9] Luo X, Yu Z, Deng C, et al. Baicalein ameliorates TNBS-induced colitis by suppressing TLR4/MyD88 signaling cascade and NLRP3 inflammasome activation in mice[J]. Sci Rep, 2017, 7(1):16374.
[10] Wei ZF, Tong B, Xia YF, et al. Norisoboldine suppresses osteoclast differentiation through preventing the accumulation of TRAF6-TAK1 complexes and activation of MAPKs/NF-κB/c-Fos/NFATc1 Pathways[J]. PLoS One, 2013, 8(3):e59171.
[11] Wei ZF, Jiao XL, Wang T, et al. Norisoboldine alleviates joint destruction in rats with adjuvant-induced arthritis by reducing RANKL, IL-6, PGE(2), and MMP-13 expression[J]. Acta Pharmacol Sin, 2013, 34(3):403-413.
[12] Luo Y, Liu M, Dai Y, et al. Norisoboldine inhibits the production of pro-inflammatory cytokines in lipopolysaccharide-sti­mu­lated RAW 264.7 cells by down-regulating the activation of MAPKs but not NF-κB[J]. Inflammation, 2010, 33(6):389-397.
[13] Tong B, Dou Y, Wang T, et al. Norisoboldine ameliorates collagen-induced arthritis through regulating the balance between Th17 and regulatory T cells in gut-associated lymphoid tissues[J]. Toxicol Appl Pharmacol, 2015, 282(1):90-99.
[14] Dinesh P, Rasool M. Berberine, an isoquinoline alkaloid suppresses TXNIP mediated NLRP3 inflammasome activation in MSU crystal stimulated RAW 264.7 macrophages through the upregulation of Nrf2 transcription factor and alleviates MSU crystal induced inflammation in rats[J]. Int Immunopharmacol, 2017, 44:26-37.
[15] Samra YA, Said HS, Elsherbiny NM, et al. Cepharanthine and piperine ameliorate diabetic nephropathy in rats:role of NF-κB and NLRP3 inflammasome[J]. Life Sci, 2016, 157:187-99.
[16] Qiu J, Wang M, Zhang J, et al. The neuroprotection of sinomenine against ischemic stroke in mice by suppressing NLRP3 inflammasome via AMPK signaling[J]. Int Immunopharmacol, 2016, 40:492-500.
[17] Liu W, Guo W, Guo L, et al. Andrographolide sulfonate ameliorates experimental colitis in mice by inhibiting Th1/Th17 response[J]. Int Immunopharmacol, 2014, 20(2):337-45.
[18] Guan Y, Tan Y, Liu W, et al. NF-E2-Related factor 2 suppresses intestinal fibrosis by inhibiting reactive oxygen species-depen­dent TGF-β1/SMADs pathway[J]. Dig Dis Sci, 2017, 63(2):366-380.
[19] Park MY, Ji GE, Sung MK. Dietary kaempferol suppresses inflammation of dextran sulfate sodium-induced colitis in mice[J]. Dig Dis Sci, 2012, 57(2):355-363.
[20] Lee SY, Lee SH, Yang EJ, et al. Coenzyme Q10 inhibits Th17 and STAT3 signaling pathways to ameliorate colitis in mice[J]. J Med Food, 2017, 20(9):821-829.
[21] Li G, Liu J, Xia WF, et al. Protective effects of ghrelin in ventilator-induced lung injury in rats[J]. Int Immunopharmacol, 2017, 52:85-91.
[22] Yuan D, Hussain T, Tan B, et al. The evaluation of antioxidant and anti-Inflammatory effects of eucommia ulmoides flavones using diquat-challenged piglet models[J]. Oxid Med Cell Longev, 2017, 2017:8140962.
[23] Xu X, Wang Y, Wei Z, et al. Madecassic acid, the contributor to the anti-colitis effect of madecassoside, enhances the shift of Th17 toward Treg cells via the PPARγ/AMPK/ACC1 pathway[J]. Cell Death Dis, 2017, 8(3):e2723.
[24] Yang N, Xia Z, Shao N, et al. Carnosic acid prevents dextran sulfate sodium-induced acute colitis associated with the regulation of the Keap1/Nrf2 pathway[J]. Sci Rep, 2017, 7(1):11036.
[25] Zhu J, Liang C, Hua Y, et al. The metastasis suppressor CD82/KAI1 regulates cell migration and invasion via inhibiting TGF-β1/Smad signaling in renal cell carcinoma[J]. Oncotarget, 2017, 8(31):51559-51568.
[26] Yang Y, Cai X, Yang J, et al. Chemoprevention of dietary digitoflavone on colitis-associated colon tumorigenesis through inducing Nrf2 signaling pathway and inhibition of inflammation[J]. Mol Cancer, 2014, 13:48.
[27] Wheeler MA, Rothhammer V, Quintana FJ. Control of immune-mediated pathology via the aryl hydrocarbon receptor[J]. J Biol Chem, 2017, 292(30):12383-12389.
[28] Martelli L, Lopez A, Strobel S, et al. Adherence to infliximab therapy in inflammatory bowel disease patients in a real-life setting[J]. J Dig Dis, 2017, 18(10):566-573­­.
[29] Maliszewska AM, Warska A, Cendrowski K, et al. Inflammatory bowel disease and pregnancy[J]. Ginekol Pol, 2017, 88(7):398-403.
[30] Galli SJ, Borregaard N, Wynn TA. Phenotypic and functional plasticity of cells of innate immunity:Macrophages, mast cells and neutrophils[J]. Nat Immunol, 2011, 12(11):1035-1044.
[31] Roberts-Thomson IC, Fon J, Uylaki W, et al. Cells, cytokines and inflammatory bowel disease:a clinical perspective[J]. Expert Rev Gastroenterol Hepatol, 2011, 5(6):703-716.
[32] Groeger D, O'Mahony L, Murphy EF, et al. Bifidobacterium infantis 35624 modulates host inflammatory processes beyond the gut[J]. Gut Microbes, 2013, 4(4):325-339.
[33] Wu P, Guo Y, Jia F, et al. The Effects of armillarisin A on serum IL-1β and IL-4 and in treating ulcerative colitis[J]. Cell Biochem Biophys, 2015, 72(1):103-106.
[34] Műzes G, Molnár B, Tulassay Z, et al. Changes of the cytokine profile in inflammatory bowel diseases[J]. World J Gastroenterol, 2012, 18(41):5848-5861.
[35] Biasi F, Leonarduzzi G, Oteiza PI, et al. Inflammatory bowel disease:mechanisms, redox considerations, and therapeutic targets[J]. Antioxid Redox Signal, 2013, 19(14):1711-1747.
[36] Netea MG, Simon A, van de Veerdonk F, et al. IL-1beta processing in host defense:beyond the inflammasomes[J]. PLoS Pathog, 2010, 6(2):e1000661.
[37] Zhang J, Fu S, Sun S, et al. Inflammasome activation plays an important role in the development of spontaneous colitis[J]. Mucosal Immunol, 2014, 7(5):1139-1150.
[38] Michail S, Bultron G, Depaolo RW. Genetic variants associated with Crohn's disease[J]. Appl Clin Genet, 2013, 16(6):25-32.
[39] Bauer C, Duewell P, Mayer C, et al. Colitis induced in mice with dextran sulfate sodium (DSS) is mediated by the NLRP3 inflammasome[J]. Gut, 2010, 59(9):1192-1199.
[40] Afonina IS, Zhong Z, Karin M, et al. Limiting inflammation-the negative regulation of NF-κB and the NLRP3 inflammasome. Nat Immunol, 2017, 18(8):861-869.
[41] Huai W, Zhao R, Song H, et al. Aryl hydrocarbon receptor negatively regulates NLRP3 inflammasome activity by inhibiting NLRP3 transcription[J]. Nat Commun, 2014, 5:4738.
[42] Benson JM, Shepherd DM. Aryl hydrocarbon receptor activation by TCDD reduces inflammation associated with Crohn's disease[J]. Toxicol Sci, 2011, 120(1):68-78.
[43] Huang Z, Jiang Y, Yang Y, et al. 3, 3'-Diindolylmethane alleviates oxazolone-induced colitis through Th2/Th17 suppression and Treg induction[J]. Mol Immunol, 2013, 53(4):335-344.
[44] Kuang H, Tang Z, Zhang C, et al. Taxifolin activates the Nrf2 anti-oxidative stress pathway in mouse skin epidermal JB6 P+ cells through epigenetic modifications[J]. Int J Mol Sci, 2017, 18(7):E1546.
[45] Davis W Jr, Ronai Z, Tew KD. Cellular thiols and reactive oxygen species in drug-induced apoptosis[J]. J Pharmacol Exp Ther, 2001, 296(1):1-6.
[46] Poprac P, Jomova K, Simunkova M, et al. Targeting free radicals in oxidative stress-related human diseases[J]. Trends Pharmacol Sci, 2017, 38(7):592-607.
[47] Abderrazak A, Syrovets T, Couchie D, et al. NLRP3 inflammasome:from a danger signal sensor to a regulatory node of oxidative stress and inflammatory diseases[J]. Redox Biol, 2015, 4:296-307.
[48] Tsai PY, Ka SM, Chang JM, et al. Epigallocatechin-3-gallate prevents lupus nephritis development in mice via enhancing the Nrf2 antioxidant pathway and inhibiting NLRP3 inflamma­some activation[J]. Free Radic Biol Med, 2011, 51(3):744-754.
[49] De S, Manna A, Kundu S, et al. Allylpyrocatechol attenuates collagen-induced arthritis via attenuation of oxidative stress secondary to modulation of the MAPK, JAK/STAT, and Nrf2/HO-1 pathways[J]. J Pharmacol Exp Ther, 2017, 360(2):249-259.
[50] Ma Q, Kinneer K, Bi Y, et al. Induction of murine NAD(P)H:quinone oxidoreductase by 2,3,7,8-tetrachlorodibenzo-p-dioxin requires the CNC (cap ‘n’ collar) basic leucine zipper transcription factor Nrf2(nuclear factor erythroid 2-related factor 2):cross-interaction between AhR (aryl hydrocarbon receptor) and Nrf2 signal transduction[J]. Biochem J, 2004, 377(Pt 1):205-213.
[51] Nakahara T, Mitoma C, Hashimoto-Hachiya A, et al. Antioxidant opuntia ficus-indica extract activates AHR-NRF2 signaling and upregulates filaggrin and loricrin expression in human keratinocytes[J]. J Med Food, 2015, 18(10):1143-1149.
[52] Esakky P, Hansen DA, Drury AM, et al. Cigarette smoke-induced cell cycle arrest in spermatocytes[GC-2spd(ts)] is mediated through crosstalk between Ahr-Nrf2 pathway and MAPK signaling[J]. J Mol Cell Biol, 2015, 7(1):73-87.