CN: 32-1845/R
ISSN: 2095-6975
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XIONG Jia, GRACE Mary H, ESPOSITO Debora, KOMARNYTSKY Slavko, WANG Fei, LILA Mary Ann. Polyphenols isolated from Acacia mearnsii bark with anti-inflammatory and carbolytic enzyme inhibitory activities[J]. 中国天然药物英文, 2017, 15(11): 816-824

Polyphenols isolated from Acacia mearnsii bark with anti-inflammatory and carbolytic enzyme inhibitory activities

XIONG Jia1,2, GRACE Mary H2, ESPOSITO Debora2, KOMARNYTSKY Slavko2, WANG Fei1, LILA Mary Ann2
1 Department of Chemical Engineering, Jiangsu Key Lab of Biomass-based Green Fuels and Chemicals, Nanjing Forestry University, Nanjing 210037, China;
2 Plants for Human Health Institute, Department of Food Bioprocessing and Nutrition Sciences, North Carolina State University, North Carolina Research Campus, Kannapolis, NC 28081, USA
摘要:
The present study was designed to characterize the polyphenols isolated from Acacia mearnsii bark crude extract (B) and fractions (B1-B7) obtained by high-speed counter-current chromatography (HSCCC) and evaluate their anti-inflammatory and carbolytic enzymes (α-glucosidase and α-amylase) inhibitory activities. Fractions B4, B5, B6, B7 (total phenolics 850.3, 983.0, 843.9, and 572.5 mg·g-1, respectively; proanthocyanidins 75.7, 90.5, 95.0, and 44.8 mg·g-1, respectively) showed significant activities against reactive oxygen species (ROS), nitric oxide (NO) production, and expression of pro-inflammatory genes interleukin-1β (IL-1β) and inducible nitric oxide synthase (iNOS) in a lipopolysaccharide (LPS)-stimulated mouse macrophage cell line RAW 264.7. All the extracts suppressed α-glucosidase and α-amylase activities, two primary enzymes responsible for carbohydrate digestion. A. mearnsii bark samples possessed significantly stronger inhibitory effects against α-glucosidase enzyme (IC50 of 0.4-1.4 μg·mL-1) than the pharmaceutical acarbose (IC50 141.8 μg·mL-1). B6 and B7 (IC5017.6 and 11.7 μg·mL-1, respectively) exhibited α-amylase inhibitory activity as efficacious as acarbose (IC50 15.4 μg·mL-1). Moreover, B extract, at 25 μg·mL-1, significantly decreased the non-mitochondrial oxidative burst that is often associated with inflammatory response in human monocytic macrophages.
关键词:    Acacia mearnsii    Proanthocyanidins    Anti-inflammatory    α-Amylase    α-Glucosidase   
收稿日期: 2016-12-24
LILA Mary Ann, Tel:+1 704.250.5407, Fax:+1 704.250.5409, E-mail:mlila@ncsu.edu
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