CN: 32-1845/R
ISSN: 2095-6975
XIONG Jia, GRACE Mary H, ESPOSITO Debora, KOMARNYTSKY Slavko, WANG Fei, LILA Mary Ann. Polyphenols isolated from Acacia mearnsii bark with anti-inflammatory and carbolytic enzyme inhibitory activities[J]. 中国天然药物英文, 2017, 15(11): 816-824

Polyphenols isolated from Acacia mearnsii bark with anti-inflammatory and carbolytic enzyme inhibitory activities

XIONG Jia1,2, GRACE Mary H2, ESPOSITO Debora2, KOMARNYTSKY Slavko2, WANG Fei1, LILA Mary Ann2
1 Department of Chemical Engineering, Jiangsu Key Lab of Biomass-based Green Fuels and Chemicals, Nanjing Forestry University, Nanjing 210037, China;
2 Plants for Human Health Institute, Department of Food Bioprocessing and Nutrition Sciences, North Carolina State University, North Carolina Research Campus, Kannapolis, NC 28081, USA
The present study was designed to characterize the polyphenols isolated from Acacia mearnsii bark crude extract (B) and fractions (B1-B7) obtained by high-speed counter-current chromatography (HSCCC) and evaluate their anti-inflammatory and carbolytic enzymes (α-glucosidase and α-amylase) inhibitory activities. Fractions B4, B5, B6, B7 (total phenolics 850.3, 983.0, 843.9, and 572.5 mg·g-1, respectively; proanthocyanidins 75.7, 90.5, 95.0, and 44.8 mg·g-1, respectively) showed significant activities against reactive oxygen species (ROS), nitric oxide (NO) production, and expression of pro-inflammatory genes interleukin-1β (IL-1β) and inducible nitric oxide synthase (iNOS) in a lipopolysaccharide (LPS)-stimulated mouse macrophage cell line RAW 264.7. All the extracts suppressed α-glucosidase and α-amylase activities, two primary enzymes responsible for carbohydrate digestion. A. mearnsii bark samples possessed significantly stronger inhibitory effects against α-glucosidase enzyme (IC50 of 0.4-1.4 μg·mL-1) than the pharmaceutical acarbose (IC50 141.8 μg·mL-1). B6 and B7 (IC5017.6 and 11.7 μg·mL-1, respectively) exhibited α-amylase inhibitory activity as efficacious as acarbose (IC50 15.4 μg·mL-1). Moreover, B extract, at 25 μg·mL-1, significantly decreased the non-mitochondrial oxidative burst that is often associated with inflammatory response in human monocytic macrophages.
关键词:    Acacia mearnsii    Proanthocyanidins    Anti-inflammatory    α-Amylase    α-Glucosidase   
收稿日期: 2016-12-24
LILA Mary Ann, Tel:+1 704.250.5407, Fax:+1 704.250.5409,
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