CN: 32-1845/R
ISSN: 2095-6975
ZHAO Wei-Man, JIANG Shu-Wen, CHEN Yang, ZHONG Ze-Yu, WANG Zhong-Jian, ZHANG Mian, LI Ying, XU Ping, LIU Li, LIU Xiao-Dong. Laminaria japonica increases plasma exposure of glycyrrhetinic acid following oral administration of Liquorice extract in rats[J]. 中国天然药物英文, 2015, 13(7): 540-549

Laminaria japonica increases plasma exposure of glycyrrhetinic acid following oral administration of Liquorice extract in rats

ZHAO Wei-Man, JIANG Shu-Wen, CHEN Yang, ZHONG Ze-Yu, WANG Zhong-Jian, ZHANG Mian, LI Ying, XU Ping, LIU Li, LIU Xiao-Dong
State Key Laboratory of Natural Medicines, Department of Complex Prescription of TCM, China Pharmaceutical University, Nanjing 211198, China
The present study was designed to investigate the effects of Laminaria japonica (Laminaria) on pharmacokinetics of glycyrrhetinic acid (GA) following oral administration of Liquorice extract in rats. Following oral administrations of single-dose and multi-dose Liquorice extract and Liquorice-Laminaria extract, respectively, plasma samples were obtained at various times and the concentrations of GA, liquiritigenin, and isoliquiritigenin were measured by LC-MS. The effects of Laminaria extract on pharmacokinetics of GA were also investigated, following single-dose and multidose of glycyrrhizic acid (GL). The effects of Laminaria extract on intestinal absorption of GA and GL were studied using the in situ single-pass intestinal perfusion model. The metabolism of GL to GA in the contents of small and large intestines was also studied. The results showed Liquorice-Laminaria extract markedly increased the plasma concentration of GA, accompanied by a shorter Tmax. Similar alteration was observed following multidose administration. However, pharmacokinetics of neither liquiritigenin nor isoliquiritigenin was affected by Laminaria. Similarly, Laminaria markedly increased concentration and decreased Tmax of GA following oral GL were observed. The data from the intestinal perfusion model showed that Laminaria markedly increased GL absorption in duodenum and jejunum, but did not affect the intestinal absorption of GA. It was found that Laminaria enhanced the metabolism of GL to GA in large intestine. In conclusion, Laminaria increased plasma exposures of GA following oral administration of liquorice or GL, which partly resulted from increased intestinal absorption of GL and metabolism of GL to GA in large intestine.
关键词:    Pharmacokinetics    Liquorice    Laminaria japonica    Glycyrrhizic acid    Glycyrrhetinic acid    In situ single-pass intestinal perfusion    Intestinal contents   
收稿日期: 2014-11-04
LIU Li, LIU Xiao-Dong
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